Analgesic Effects of Fatty Acid Amide Hydrolase Inhibition in a Rat Model of Neuropathic Pain

• DOI: 10.1523/jneurosci.3326-06.2006
• Publication Date: 2006-12-20T17:28:11Z
• Authors: Maulik D. Jhaveri; Denise Richardson; David A. Kendall; David A. Barrett; Victoria Chapman
• Keywords: Anandamide; AM251

Cannabinoid-based medicines have therapeutic potential for the treatment of pain. Augmentation levels endocannabinoids with inhibitors fatty acid amide hydrolase (FAAH) is analgesic in models acute and inflammatory pain states. The aim this study was to determine whether local inhibition FAAH alters nociceptive responses spinal neurons nerve ligation model neuropathic Electrophysiological studies were performed 14–18 d after or sham surgery, effects inhibitor cyclohexylcarbamic 3-carbamoyl biphenyl-3-yl ester (URB597) on mechanically evoked determined. Intraplantar URB597 (25 μg 50 μl) significantly ( p < 0.01) attenuated sham-operated rats. Effects blocked by cannabinoid 1 receptor (CB ) antagonist AM251 [ N -1-(2,4-dichlorophenyl)-5-(4-iodophenyl)-4-methyl- -1-piperidinyl-1 H -pyrazole-3-carboxamide] (30 opioid naloxone. increased anandamide, 2-arachidonyl glycerol, oleoyl ethanolamide ipsilateral hindpaw did not, however, alter ligated (SNL) rats endocannabinoids. injection a higher dose (100 0.05) SNL but not Spinal administration elevated These data suggest that peripheral activity may be altered alternative pathways metabolism greater importance