LRRK2 Controls Synaptic Vesicle Storage and Mobilization within the Recycling Pool

• DOI: 10.1523/jneurosci.3730-10.2011
• Publication Date: 2011-02-09T17:27:51Z
• Authors: Giovanni Piccoli; Steven B. Condliffe; Matthias Bauer; Florian Giesert; Karsten Boldt; Silvia De Astis; Andrea Meixner; Hakan Sarioglu; Daniela M. Vogt-W...; Wolfgang Wurst; Christian Johanne...; Michela Matteoli; Carlo Sala; Marius Ueffing
• Keywords: LRRK2

Mutations in leucine-rich repeat kinase 2 ( LRRK2 ) are the single most common cause of inherited Parkinson's disease. Little is known about its involvement pathogenesis disease mainly because lack knowledge physiological role LRRK2. To determine function LRRK2, we studied impact short hairpin RNA-mediated silencing expression cortical neurons. Paired recording indicated that affects evoked postsynaptic currents. Furthermore, induces at presynaptic site a redistribution vesicles within bouton, altered recycling dynamics, and increased vesicle kinetics. Accordingly, protein present synaptosomal compartment neurons which it interacts with several proteins involved vesicular recycling. Our results suggest modulates synaptic trafficking distribution consequence participates regulating dynamics between pools inside bouton.