A Critical Role for Purinergic Signalling in the Mechanisms Underlying Generation of BOLD fMRI Responses

• DOI: 10.1523/jneurosci.3787-14.2015
• Publication Date: 2015-04-01T16:52:02Z
• Authors: Jack A. Wells; Isabel N. Christie; Patrick S. Hosford; Robert T.R. Hucks...; Plamena R. Angelova; Pirkko Vihko; Simon C. Cork; Andrey Y. Abramov; Anja G. Teschemacher; Sergey Kasparov; Mark F. Lythgoe; Alexander V. Gourine
• Keywords: Male; 570; nervous-system; Microinjections; glia; cerebral-blood-flow; somatosensory cortex; fmri; neurovascular coupling; Acid Phosphatase; 610; mouse cortex; bold; alpha-chloralose; atp; 03 medical and health sciences; astrocyte; Adenosine Triphosphate; neurosciences & neurology; Forelimb; Animals; 0303 health sciences; atp release.; Functional Neuroimaging; neurosciences; functional activation; calcium dynamics; Somatosensory Cortex; life sciences & biomedicine; Magnetic Resonance Imaging; Electric Stimulation; Rats; Purinergic P1 Receptor Antagonists; cortex in-vivo; Cerebrovascular Circulation; prostatic-acid-phosphatase; Protein Tyrosine Phosphatases; Science & technology; Signal Transduction

The mechanisms of neurovascular coupling underlying generation of BOLD fMRI signals remain incompletely understood. It has been proposed that release of vasoactive substances by astrocytes couples neuronal activity to changes in cerebrovascular blood flow. However, the role of astrocytes in fMRI responses remains controversial. Astrocytes communicate via release of ATP, and here we tested the hypothesis that purinergic signaling plays a role in the mechanisms underlying fMRI. An established fMRI paradigm was used to trigger BOLD responses in the forepaw region of the somatosensory cortex (SSFP) of an anesthetized rat. Forepaw stimulation induced release of ATP in the SSFP region. To interfere with purinergic signaling by promoting rapid breakdown of the vesicular and/or released ATP, a lentiviral vector was used to express a potent ectonucleotidase, transmembrane prostatic acid phosphatase (TMPAP), in the SSFP region. TMPAP expression had no effect on resting cerebral blood flow, cerebrovascular reactivity, and neuronal responses to sensory stimulation. However, TMPAP catalytic activity markedly reduced the magnitude of BOLD fMRI responses triggered in the SSFP region by forepaw stimulation. Facilitated ATP breakdown could result in accumulation of adenosine. However, blockade of A1receptors had no effect on BOLD responses and did not reverse the effect of TMPAP. These results suggest that purinergic signaling plays a significant role in generation of BOLD fMRI signals. We hypothesize that astrocytes activated during periods of enhanced neuronal activity release ATP, which propagates astrocytic activation, stimulates release of vasoactive substances and dilation of cerebral vasculature.