In VivoQuantification of Myelin Changes in the Vertebrate Nervous System

• DOI: 10.1523/jneurosci.4082-08.2009
• Publication Date: 2009-11-19T00:01:28Z
• Authors: Yanming Wang; Chunying Wu; Andrew V. Caprari...; Eduardo Somoza; Wenxia Zhu; Changning Wang; Robert H. Miller
• Keywords: Remyelination; Molecular Imaging

Destruction or changes associated with myelin membranes in the CNS play a key role pathogenesis of multiple sclerosis and other related neurodegenerative disorders. A long-standing goal has been to detect quantify content vivo . For this reason, we have developed myelin-imaging technique based on positron emission tomography (PET). PET is quantitative imaging modality that widely used clinical settings for direct assessment biological processes at molecular level. However, lack probes hampered use myelination CNS. Here, report agent, termed Case Imaging Compound (CIC) readily penetrates blood–brain barrier preferentially localizes myelinated regions brain. After radiolabeling positron-emitting carbon-11, [ 11 C]CIC–PET was conducted longitudinal studies using lysolethicin-induced rat model focal demyelination subsequent remyelination. Quantitative analysis showed retention C]CIC correlates level demyelination/remyelination. These indicate that, first time, can be as an marker myelination, which potential translated into myelin-related diseases early diagnosis, subtyping, efficacy evaluation therapeutic treatments aimed repair.