Genetic Fate Mapping Reveals That the Caudal Ganglionic Eminence Produces a Large and Diverse Population of Superficial Cortical Interneurons

• DOI: 10.1523/jneurosci.4515-09.2010
• Publication Date: 2010-02-03T19:29:38Z
• Authors: Goichi Miyoshi; Jens Hjerling-Lef...; Theofanis Karayannis; Vitor H. Sousa; Simon J. B. Butt; James Battiste; Jane E. Johnson; Robert P. Machold; Gord Fishell
• Keywords: Ganglionic eminence; Interneuron

By combining an inducible genetic fate mapping strategy with electrophysiological analysis, we have systematically characterized the populations of cortical GABAergic interneurons that originate from caudal ganglionic eminence (CGE). Interestingly, compared medial (MGE)-derived interneuron populations, initiation [embryonic day 12.5 (E12.5)] and peak production (E16.5) this embryonic structure occurs 3 d later in development. Moreover, unlike either pyramidal cells or MGE-derived interneurons, CGE-derived do not integrate into cortex inside-out manner but preferentially (75%) occupy superficial layers independent birthdate. In contrast to previous estimates, are both considerably greater number (∼30% all interneurons) diversity (comprised by at least nine distinct subtypes). Furthermore, found a large proportion including neurogliaform subtype, express glycoprotein Reelin. fact, most Reelin vasoactive intestinal polypeptide. Thus, conjunction studies, now determined spatial temporal origins vast majority subtypes.