Kainate Receptors Mediate Signaling in Both Transient and Sustained OFF Bipolar Cell Pathways in Mouse Retina

• DOI: 10.1523/jneurosci.4941-13.2014
• Publication Date: 2014-04-30T16:37:52Z
• Authors: Bart G. Borghuis; Loren L. Looger; Susumu Tomita; Jonathan B. Demb
• Keywords: Kainate receptor; Long-term depression; Silent synapse

A fundamental question in sensory neuroscience is how parallel processing implemented at the level of molecular and circuit mechanisms. In retina, it has been proposed that distinct OFF cone bipolar cell types generate fast/transient slow/sustained pathways by differential expression AMPA- kainate-type glutamate receptors, respectively. However, functional significance these receptors intact during light stimulation remains unclear. Here, we measured release from mouse cells two-photon imaging a sensor (iGluSnFR) expressed on postsynaptic amacrine ganglion dendrites. both transient sustained layers, cone-driven was blocked antagonists to kainate but not AMPA receptors. Electrophysiological recordings confirmed essential role for signaling pathways. Kainate mediated responses contrast modulation up 20 Hz. Light-evoked all depend kainate, AMPA,