Kisspeptin Signaling Is Required for Peripheral But Not Central Stimulation of Gonadotropin-Releasing Hormone Neurons by NMDA

• DOI: 10.1523/jneurosci.5486-09.2010
• Publication Date: 2010-06-23T16:53:24Z
• Authors: X. d'Anglemont de...; K. J. Ackroyd; E. E. Chatzidaki; W. H. Colledge
• Keywords: Kisspeptin; Neurokinin B

NMDA and kisspeptins can stimulate gonadotropin-releasing hormone (GnRH) release after peripheral or central administration in mice. To determine whether these agonists act independently through a common pathway, we have examined their ability to GnRH/luteinizing (LH) Kiss1- Gpr54 (Kiss1r)-null mutant Peripheral injection of failed GnRH/LH prepubertal gonadally intact male Dual-labeling experiments indicated direct activation Kiss1-expressing neurons the arcuate nucleus. In contrast, into lateral ventricle increased plasma LH levels both Kiss1 mice similar responses wild-type Central stimulated c-Fos expression throughout hypothalamus but not GnRH neurons, suggesting an action at nerve terminals only. kisspeptin-10 induced only administration. Finally, induces catecholamine- nitric oxide-producing mice, indicating possible kisspeptin-independent by neurons. Thus, may cell bodies (kisspeptin-independent) (kisspeptin-dependent) dual way participate secretion mouse.