A dual‐function RNA balances carbon uptake and central metabolism in Vibrio cholerae

• DOI: 10.15252/embj.2021108542
• Publication Date: 2021-10-08T17:32:25Z
• Authors: Kavyaa Venkat; Mona Hoyos; James RJ Haycocks; Liam Cassidy; Beatrice Engelmann; Ulrike Rolle‐Kamp...; Martin von Bergen; Andreas Tholey; David C Grainger; Kai Papenfort
• Keywords: Dual function

Bacterial small RNAs (sRNAs) are well known to modulate gene expression by base pairing with trans-encoded transcripts and typically non-coding. However, several sRNAs have been reported also contain an open reading frame thus considered dual-function RNAs. In this study, we discovered a RNA from Vibrio cholerae, called VcdRP, harboring 29 amino acid protein (VcdP), as base-pairing sequence. Using forward genetic screen, identified VcdRP repressor of cholera toxin production link phenotype the inhibition carbon transport segment regulator. By contrast, demonstrate that VcdP acts downstream binding citrate synthase (GltA), first enzyme citric cycle. Interaction GltA results in increased activity together VcdR reroute metabolism. We further show transcription vcdRP is repressed CRP allowing us provide model which employs two different molecular mechanisms synchronize central metabolism V. cholerae.