The ribosome‐associated chaperone Zuo1 controls translation upon TORC1 inhibition

• DOI: 10.15252/embj.2022113240
• Publication Date: 2023-11-21T17:03:58Z
• Authors: Ailsa Black; Thomas D Williams; Flavie Soubigou; Ifeoluwapo M Joshua; Houjiang Zhou; Frederic Lamoliatte; Adrien Rousseau
• Keywords: Chaperone (clinical)

Protein requirements of eukaryotic cells are ensured by proteostasis, which is mediated tight control TORC1 activity. Upon inhibition, protein degradation increased and synthesis reduced through inhibition translation initiation to maintain cell viability. Here, we show that the ribosome-associated complex (RAC)/Ssb chaperone system, composed HSP70 Ssb its HSP40 co-chaperone Zuo1, required proteostasis viability under in Saccharomyces cerevisiae. In absence does not decrease response loss A functional interaction between Zuo1 for proper translational maintenance upon inhibition. Furthermore, have shown rapid eIF4G following autophagy prevented zuo1Δ cells, contributing decreased survival these conditions. We found defective impedes Our findings identify an essential role RAC/Ssb regulating changes signalling.