Extracellular vesicles and co‐isolated endogenous retroviruses from murine cancer cells differentially affect dendritic cells

• DOI: 10.15252/embj.2023113590
• Publication Date: 2023-12-11T09:23:03Z
• Authors: Federico Cocozza; Lorena Martin‐Jaular; Lien Lippens; Aurelie Di Cicco; Yago A Arribas; Nicolas Ansart; Florent Dingli; Michael Richard; Louise Merle; Mabel Jouve San R...; Patrick Poullet; Damarys Loew; Daniel Lévy; An Hendrix; George Kassiotis; Alain Joliot; Mercedes Tkach; Clotilde Théry
• Keywords: Extracellular vesicles; Priming (agriculture); Extracellular Vesicles

Abstract Cells secrete extracellular vesicles (EVs) and non‐vesicular (nano)particles (NVEPs or ENPs) that may play a role in intercellular communication. Tumor‐derived EVs have been proposed to induce immune priming of antigen presenting cells be immuno‐suppressive agents. We suspect such disparate functions are due variable compositions EV subtypes ENPs. aimed characterize the array secreted ENPs murine tumor cell lines. Unexpectedly, we identified virus‐like particles (VLPs) from endogenous leukemia virus preparations produced by many cells. established protocol separate small VLPs compared their protein composition analyzed functional interaction with target dendritic were poorly captured did not affect Small specifically induced death. A mixed large/dense EV/VLP preparation was most efficient maturation presentation. Our results call for systematic re‐evaluation respective proportions non‐viral tumors contribution progression.