Optimising the preparation method of seeds of Agriophyllum squarrosum prior to staining in tetrazolium solution

• DOI: 10.15258/sst.2014.42.2.19
• Publication Date: 2014-09-02T09:51:28Z
• Authors: Y.J. Zeng; X.N. Qi; Y.Z. Li; Y.Q. Wang; Y.R. Wang
• Keywords: Tetrazolium chloride; Formazan

Seven samples of Agriophyllum squarrosum seeds were obtained from Minqin County Gansu province, China. These prepared for tetrazolium testing through three approaches, namely, keeping the seed intact, piercing centre and cutting basal half longitudinally. Viability was also assessed a germination test. Prior to or cutting, should be pre-moistened in 20 degrees C water at least 24 hours. Intact unsuitable because most them remained unstained after 30 hours solution. Piercing expose embryo required similar optimum staining time (10 hours) resulted effect viability percentage. Nonetheless, recommended as tissue exposure method over latter is time-consuming may cause damage embryo. All seven exhibited high (97.8-99.8%). Four displayed similarly (93.3-98.8%), whereas other significantly different (89.8-50.0%) viabilities recorded (P < 0.05, N = 4). An procedure A. proposed.