Melatonin inhibits IL1β-induced MMP9 expression and activity in human umbilical vein endothelial cells by suppressing NF-κB activation

• DOI: 10.1530/joe-12-0147
• Publication Date: 2012-05-23T02:51:51Z
• Authors: Weiwei Qin; Wenbao Lu; Hongwei Li; Xiaochen Yuan; Bingwei Li; Qiuju Zhang; Ruijuan Xiu
• Keywords: MMP9; Occludin

Matrix metalloproteinases (MMPs) have been involved in inflammatory and degradative processes pathologic conditions. The purpose of this study was to investigate the protective effect melatonin human umbilical vein endothelial cell (HUVEC) monolayer permeability regulation MMP9 induced by interleukin 1β (IL1β (IL1B)) HUVECs. Protection studies were carried out with melatonin, a well-known antioxidant antiinflammatory molecule. expression increased IL1β induction Melatonin showed barrier-protective role downregulation upregulation tissue inhibitor metalloproteinase-1 Meanwhile, also decreased sodium fluorescein counteracted vascular cadherin occludin During stimulus, nuclear factor-κB (NF-κB) plays significant regulating MMP genes expression, thus function NF-κB HUVECs' barrier disruption investigated. translocation HUVECs regulated expression. However, into nucleus inhibited significantly melatonin. Our results show that decreases IL1β. At same time, activity NF-κB-dependent pathway