Gliquidone decreases urinary protein by promoting tubular reabsorption in diabetic Goto-Kakizaki rats

• DOI: 10.1530/joe-13-0199
• Publication Date: 2013-11-20T05:17:53Z
• Authors: Jian-Ting Ke; Mi Li; Shi-Qing Xu; Wen-Jian Zhang; Yong-Wei Jiang; Lan-yun Cheng; Li Chen; Jin-Ning Lou; Wei Wu
• Keywords: Male; 0303 health sciences; Drug Evaluation, Preclinical; Rats, Inbred Strains; Absorption; Diabetes Mellitus, Experimental; Rats; 3. Good health; Proteinuria; 03 medical and health sciences; Kidney Tubules; Sulfonylurea Compounds; Animals; Humans; Hypoglycemic Agents; Diabetic Nephropathies; Cells, Cultured

The efficacy of gliquidone for the treatment diabetic nephropathy was investigated by implanting micro-osmotic pumps containing into abdominal cavities Goto-Kakizaki (GK) rats with nephropathy. Blood glucose, 24 h urinary protein, and albumin levels were measured weekly. After 4 weeks therapy, pathological changes in glomerular basement membrane (GBM) examined using an electron microscope. Real-time PCR, western blotting, immunohistochemistry employed to detect expression receptors advanced glycation end products (RAGE) (AGER), protein kinase C β (PKCβ), A (PKA) as well tubular reabsorption-associated proteins: megalin cubilin. Human proximal epithelial cells (HK-2 cells) used analyze effects (AGEs) on cubilin absorption albumin. Gliquidone lowered blood GK level plasma C-peptide increased markedly GBM podocyte lesions improved dramatically after treatment. Glomerular RAGE PKCβ decreased treatment, while PKA increased. AGEs suppressed cubulin HK-2 vitro , whereas all these In conclusion, effectively reduced improving promoting reabsorption.