Signaling pathways regulating FSH- and amphiregulin-induced meiotic resumption and cumulus cell expansion in the pig

• DOI: 10.1530/rep-12-0191
• Publication Date: 2012-09-05T06:04:15Z
• Authors: R Prochazka; M Blaha; L Nemcova
• Keywords: Germinal vesicle; LY294002; Wortmannin

To define signaling pathways that drive FSH- and epidermal growth factor (EGF)-like peptide-induced cumulus expansion oocyte meiotic resumption, in vitro cultured pig cumulus-oocyte complexes were treated with specific protein kinase inhibitors. We found FSH-induced maturation of oocytes was blocked germinal vesicle (GV) stage by A (PKA), MAPK14, MAPK3/1, EGF receptor (EGFR) tyrosine inhibitors (H89, SB203580, U0126, AG1478 respectively) whereas phosphoinositide-3-kinase/v-akt murine thymoma viral oncogene homolog (PI3K/AKT) inhibitor (LY294002) metaphase I (MI). Amphiregulin (AREG)-induced efficiently GV AG1478, low concentrations LY294002; H89, high LY294002 allowed the to undergo breakdown MI. Both AREG-induced incompletely inhibited H89 completely LY294002. The partially or expression expansion-related genes (HAS2, PTGS2, TNFAIP6) two exceptions: only TNFAIP6 increased PTGS2. results this study are consistent idea PKA MAPK14 essential for transactivation EGFR, synthesis EGF-like peptides cells MAPK3/1 is involved regulation transcriptional posttranscriptional events required resumption expansion. PI3K/AKT important expansion, MI/MII transition. present data also indicate existence an FSH-activated PKA-independent pathway HAS2 PTGS2 cells.