Role of macrophage secretions on rat polycystic ovary: its effect on apoptosis

• DOI: 10.1530/rep-15-0216
• Publication Date: 2015-08-12T02:09:50Z
• Authors: Florencia Figueroa; Alicia Motta; Mariano Acosta; Fabian Mohamed; Liliana Oliveros; Myriam Forneris
• Keywords: Blotting, Western; Nitric Oxide Synthase Type II; Apoptosis; Nitric Oxide; Real-Time Polymerase Chain Reaction; PROTAGLANDIN E2; Dinoprostone; Immunoenzyme Techniques; ANDROGENS; 03 medical and health sciences; Contraceptive Agents; https://purl.org/becyt/ford/1.6; Animals; RNA, Messenger; MACROPHAGES; https://purl.org/becyt/ford/1; Cells, Cultured; Cell Proliferation; 0303 health sciences; Estradiol; Macrophages; Androstenedione; APOPTOSIS; Rats; Disease Models, Animal; Proto-Oncogene Proteins c-bcl-2; POLYCYSTIC OVARY; Female; Polycystic Ovary Syndrome

Polycystic ovarian syndrome is the most common endocrine disorder among women of reproductive age. Little known about its etiology, although evidence suggests an intrinsic abnormality in which endocrine, metabolic, neural and immune factors would be involved. In this work, effects macrophage (MO) secretion on apoptosis a polycystic ovary rat model (PCO rat) induced by estradiol valerate are studied. Spleen MO secretions were used to stimulate ovaries interstitial granulosa cells from both PCO control rats. Ovarian hormones prostaglandin E2 (PGE2) measured RIA; mRNA levels Bax, Bcl2 NFkB RT-PCR; inducible nitric oxide synthase (iNOS) western blot. The number apoptotic was evaluated TUNEL. ovary, rats increased Bax expressions TUNEL staining theca cells. addition, produced decrease release, iNOS protein level PGE2 content it also increase androstenedione production cells, comparison with secretions. Considering these results knowing that testosterone stimulates tumour necrosis factor-α modifying response increasing androstenedione, reasonable suggest androgens stimulated could turn cytokine MO, thus maintaining vicious cycle ovary.