End-Joining Repair of Double-Strand Breaks in Drosophila melanogaster Is Largely DNA Ligase IV Independent

• DOI: 10.1534/genetics.104.033902
• Publication Date: 2004-12-20T17:12:40Z
• Authors: Mitch McVey; Dora Radut; Jeff J Sekelsky
• Keywords: Non-homologous end joining; Ku80

Abstract Repair of DNA double-strand breaks can occur by either nonhomologous end joining or homologous recombination. Most requires a specialized ligase, ligase IV (Lig4). In Drosophila melanogaster, created excision P element are usually repaired recombination pathway called synthesis-dependent strand annealing (SDSA). SDSA invasion mediated DmRad51, the product spn-A gene. mutants, repair proceeds through nonconservative involving microhomologies found within 17-nt overhangs produced excision. We report here that P-element in absence DmRad51 does not require LIG4. wild-type flies, is sometimes incomplete, and finished an end-joining also appears to be independent Loss LIG4 increase sensitivity ionizing radiation late-stage larvae, but lig4 double mutants do show heightened relative single mutants. Together, our results suggest LIG4-independent responsible for majority break flies.