Antagonizing Scalloped With a Novel Vestigial Construct Reveals an Important Role for Scalloped in Drosophila melanogaster Leg, Eye and Optic Lobe Development

• DOI: 10.1534/genetics.106.063966
• Publication Date: 2006-11-17T01:45:33Z
• Authors: Ankush Garg; Ajay Srivastava; Monica M Davis; Sandra L O'Keefe; Leola Chow; John B Bell
• Keywords: Melanogaster

Abstract Scalloped (SD), a TEA/ATTS-domain-containing protein, is required for the proper development of Drosophila melanogaster. Despite being expressed in variety tissues, most work on SD has been restricted to understanding its role and function patterning adult wing. To gain better development, we generated sd47M flip-in mitotic clones. The clones had developmental defects leg eye. Further, by removing VG domains involved activation, created reagent (VGΔACT) that disrupts ability form functional transcription factor complex produced similar phenotypes VGΔACT construct also disrupted CNS development. Expression wing alters cellular localization produces mutant phenotype, indicating able antagonize normal SD/VG complex. protein:protein interaction portion elicit phenotypes, suggesting interacts with other cofactors leg, eye, CNS. Furthermore, antagonizing larval tissues results cell death, may have survival.