Transposition of a Fungal Miniature Inverted-Repeat Transposable Element Through the Action of a Tc1-Like Transposase

• DOI: 10.1534/genetics.106.064360
• Publication Date: 2006-12-19T01:48:45Z
• Authors: Marie Dufresne; Aurélie Hua-Van; Hala Abd el Wahab; Sarrah Ben M'Barek; Christelle Vasnier; Laure Teysset; Gert H J Kema; Marie-Josée Dabo...
• Keywords: Transposase; Inverted repeat; Transposition (logic); Insertion sequence; P element

The mimp1 element previously identified in the ascomycete fungus Fusarium oxysporum has hallmarks of miniature inverted-repeat transposable elements (MITEs): short size, terminal inverted repeats (TIRs), structural homogeneity, and a stable secondary structure. Since no coding capacity, its mobilization requires transposase-encoding element. On basis similarity TIRs target-site preference with autonomous Tc1-like impala, together correlated distribution both among genus, we investigated ability to jump upon expression impala transposase provided trans. Under these conditions, present evidence that transposes by cut-and-paste mechanism into TA dinucleotides, which are duplicated insertion. Our results also show reinserts very frequently genic regions for at least one-third cases. We mimp1/impala double-component system is fully functional heterologous species F. graminearum, allowing development highly efficient tool gene tagging filamentous fungi.