Thirty-One Flavors of Drosophila Rab Proteins

• DOI: 10.1534/genetics.106.066761
• Publication Date: 2007-04-05T00:12:59Z
• Authors: Jun Zhang; Karen L Schulze; P Robin Hiesinger; Kaye Suyama; Stream Wang; Matthew Fish; Melih Acar; Roger A Hoskins; Hugo J Bellen; Matthew P Scott
• Keywords: Rab; Vesicular Transport Proteins

Rab proteins are small GTPases that play important roles in transport of vesicle cargo and recruitment, association motor other with vesicles, docking fusion vesicles at defined locations. In vertebrates, >75 genes have been identified, some which intensively studied for their endosome synaptic trafficking. Recent studies the functions certain revealed specific mediating developmental signal transduction. We begun a systematic genetic study 33 Drosophila. Most fly clearly related to vertebrate proteins. report here creation set transgenic lines allow spatially temporally regulated expression Drosophila generated fluorescent protein-tagged wild-type, dominant-negative, constitutively active forms 31 describe patterns during embryogenesis, subcellular localization proteins, comparisons selected The high evolutionary conservation low redundancy make these useful tool kit investigating vivo.