The Blast Resistance Gene Pi37 Encodes a Nucleotide Binding Site–Leucine-Rich Repeat Protein and Is a Member of a Resistance Gene Cluster on Rice Chromosome 1

• DOI: 10.1534/genetics.107.080648
• Publication Date: 2007-10-19T01:07:04Z
• Authors: Fei Lin; Shen Chen; Zhiqun Que; Ling Wang; Xinqiong Liu; Qinghua Pan
• Keywords: Pseudogene; R gene; Gene cluster

Abstract The resistance (R) gene Pi37, present in the rice cultivar St. No. 1, was isolated by an silico map-based cloning procedure. equivalent genetic region Nipponbare contains four nucleotide binding site–leucine-rich repeat (NBS–LRR) type loci. These candidates for Pi37 (Pi37-1, -2, -3, and -4) were amplified separately from 1 via long-range PCR, cloned into a binary vector. Each construct individually transformed highly blast susceptible Q1063. subsequent complementation analysis revealed Pi37-3 to be functional gene, while -1, -4 are probably pseudogenes. encodes 1290 peptide NBS–LRR product, presence of substitutions at two sites NBS (V239A I247M) is associated with phenotype. Semiquantitative expression showed that constitutively expressed only slightly induced infection. Transient experiments indicated product restricted cytoplasm. thought have evolved recently which turn derived ancestral -1 sequence. Pi37-4 likely most member cluster represents duplication -3. paralogs more closely related maize rp1 than any currently R genes Pita, Pib, Pi9, Pi2, Piz-t, Pi36.