Investigating the Genetic Circuitry of Mastermind in Drosophila, a Notch Signal Effector

• DOI: 10.1534/genetics.107.080994
• Publication Date: 2007-12-12T04:46:15Z
• Authors: Mark W Kankel; Gregory D Hurlbut; Geeta Upadhyay; Vijay Yajnik; Barry Yedvobnick; Spyros Artavanis-...
• Keywords: Cyclin-dependent kinase 8; Genetic screen; Hes3 signaling axis; Notch proteins

Abstract Notch signaling regulates multiple developmental processes and is implicated in various human diseases. Through use of the transcriptional co-activator mastermind, we conducted a screen for signal modifiers using Exelixis collection insertional mutations, which affects ∼50% Drosophila genome, recovering 160 genes never before associated with Notch, extending previous roster that interact functionally pathway mastermind. As molecular identity most recovered known, gene ontology (GO) analysis was applied, grouping according to functional classifications. We identify novel Notch-associated GO categories, uncover nodes integration between other pathways, unveil groups suggest existence Notch-independent mastermind functions, including conserved ability regulate Wnt signaling.