A SUMO-Like Domain Protein, Esc2, Is Required for Genome Integrity and Sister Chromatid Cohesion in Saccharomyces cerevisiae

• DOI: 10.1534/genetics.107.086249
• Publication Date: 2008-09-01T00:12:39Z
• Authors: Tomoko Ohya; Hirokazu Arai; Yoshino Kubota; Hideo Shinagawa; Takashi Hishida
• Keywords: DNA Replication; 0301 basic medicine; Saccharomyces cerevisiae Proteins; Models, Genetic; Recombinant Fusion Proteins; Cell Cycle; Nuclear Proteins; Cell Cycle Proteins; Saccharomyces cerevisiae; Chromatids; 03 medical and health sciences; Chromosome Segregation; Two-Hybrid System Techniques; Hydroxyurea; Gene Silencing; Genome, Fungal; Gene Deletion; DNA Damage; Plasmids

The ESC2 gene encodes a protein with two tandem C-terminal SUMO-like domains and is conserved from yeasts to humans. Previous studies have implicated Esc2 in silencing. Here, we explore the functional significance of describe novel role for promoting genome integrity during DNA replication. This study shows that esc2Delta cells are modestly sensitive hydroxyurea (HU) defective sister chromatid cohesion reduced life span, these effects enhanced by deletion RRM3 Pif1-like helicase. rrm3Delta also severe growth defect accumulate damage late S/G2. In contrast, does not enhance HU sensitivity or mrc1Delta cells, but rather partially suppresses both phenotypes. We show destabilizes functionally inactivates Esc2, this phenotype suppressed an variant authentic SUMO domain. These results suggest equivalent stable fusion plays important roles facilitating replication fork progression would otherwise impede cells.