Organization of the Synaptonemal Complex During Meiosis in Caenorhabditis elegans

• DOI: 10.1534/genetics.111.132431
• Publication Date: 2011-08-13T00:16:57Z
• Authors: Kristina Schild-P...; Takamune T Saito; Sarit Smolikov; Yanjie Gu; Marina Hincapie; David E Hill; Marc Vidal; Kent McDonald; Monica P Colaiácovo
• Keywords: Synapsis; Synaptonemal complex

Abstract Four different SYP proteins (SYP-1, SYP-2, SYP-3, and SYP-4) have been proposed to form the central region of synaptonemal complex (SC) thereby bridging axes paired meiotic chromosomes in Caenorhabditis elegans. Their interdependent localization suggests that they may interact within SC. Our studies reveal for first time how these are organized Yeast two-hybrid co-immunoprecipitation show SYP-1 is only protein capable homotypic interactions, able with both SYP-2 SYP-3 directly, whereas do not seem each other. Specifically, coiled-coil domain required its interactions interaction C-terminal SYP-2. Meanwhile, interacts end via N-terminal domain. Immunoelectron microscopy analysis provides insight into orientation While localizes close proximity chromosome axes, domains SYP-4, as well located middle Taking account sizes proteins, their abilities, SC, we propose a model link homologous provide conserved structure width SC C.