The Novel Secreted Factor MIG-18 Acts with MIG-17/ADAMTS to Control Cell Migration in Caenorhabditis elegans

• DOI: 10.1534/genetics.113.157685
• Publication Date: 2013-12-07T05:24:54Z
• Authors: Hon-Song Kim; Yuko Kitano; Masataka Mori; Tomomi Takano; Thomas Edward Har...; Kae Mizutani; Haruka Yanagimoto; Sayaka Miwa; Shinji Ihara; Yukihiko Kubota; Yukimasa Shibata; Kohji Ikenishi; Gian Garriga; Kiyoji Nishiwaki
• Keywords: ADAMTS; Fibulin

Abstract The migration of Caenorhabditis elegans gonadal distal tip cells (DTCs) offers an excellent model to study the epithelial tubes in organogenesis. mig-18 mutants cause meandering or wandering DTCs during gonad formation, which is very similar that observed animals with mutations mig-17, encodes a secreted metalloprotease ADAMTS (a disintegrin and thrombospondin motifs) family. MIG-18 novel protein conserved only among nematode species. mig-17(null) double exhibited phenotypes those single mutants. In addition, fbl-1/fibulin-1 let-2/collagen IV suppress mig-17 also suppressed mutation, suggesting function common genetic pathway. Venus-MIG-18 fusion was from muscle localized basement membrane, tissue distribution reminiscent for MIG-17. Overexpression vice versa partially rescued relevant DTC defects, MIG-17 act cooperatively rather than sequentially. We propose may be cofactor MIG-17/ADAMTS functions regulation membrane achieve proper direction gonadogenesis.