Mapping-by-Sequencing IdentifiesHvPHYTOCHROME Cas a Candidate Gene for theearly maturity 5Locus Modulating the Circadian Clock and Photoperiodic Flowering in Barley

• DOI: 10.1534/genetics.114.165613
• Publication Date: 2014-07-05T01:44:07Z
• Authors: Artem Pankin; Chiara Campoli; Xue Dong; Benjamin Kilian; Rajiv Sharma; Axel Himmelbach; Reena Saini; Seth J Davis; Nils Stein; Korbinian Schneeb...; Maria von Korff
• Keywords: 0303 health sciences; Photoperiod; Quantitative Trait Loci; Plant Development; Hordeum; Flowers; Investigations; Genes, Plant; Physical Chromosome Mapping; 03 medical and health sciences; Circadian Clocks; Exome; Inbreeding; Phytochrome; Plant Proteins

AbstractPhytochromes play an important role in light signaling and photoperiodic control of flowering time in plants. Here we propose that the red/far-red light photoreceptor HvPHYTOCHROME C (HvPHYC), carrying a mutation in a conserved region of the GAF domain, is a candidate underlying the early maturity 5 locus in barley (Hordeum vulgare L.). We fine mapped the gene using a mapping-by-sequencing approach applied on the whole-exome capture data from bulked early flowering segregants derived from a backcross of the Bowman(eam5) introgression line. We demonstrate that eam5 disrupts circadian expression of clock genes. Moreover, it interacts with the major photoperiod response gene Ppd-H1 to accelerate flowering under noninductive short days. Our results suggest that HvPHYC participates in transmission of light signals to the circadian clock and thus modulates light-dependent processes such as photoperiodic regulation of flowering.