Replisome Function During Replicative Stress Is Modulated by Histone H3 Lysine 56 Acetylation Through Ctf4

• DOI: 10.1534/genetics.114.173856
• Publication Date: 2015-02-20T20:16:32Z
• Authors: Pierre Luciano; Pierre-Marie Dehé; Stéphane Audebert; Vincent Géli; Yves Corda
• Keywords: Replisome

Abstract Histone H3 lysine 56 acetylation in Saccharomyces cerevisiae is required for the maintenance of genome stability under normal conditions and upon DNA replication stress. Here we show that absence replisome components become deleterious when forks collapse at natural block sites. This lethality not a direct consequence chromatin assembly defects during fork progression. Rather, our genetic analyses suggest presence replicative stress uncouples Cdc45–Mcm2-7–GINS helicase complex polymerases through component Ctf4. In addition, discovered N-terminal domain Ctf4, necessary interaction Ctf4 with Mms22, an adaptor protein Rtt101-Mms1 E3 ubiquitin ligase, function pathway, suggesting promotes between Mms22. Taken together, results indicate essential member pathway provide novel mechanistic insights into understanding role maintaining