Adult Muscle Formation Requires Drosophila Moleskin for Proliferation of Wing Disc-Associated Muscle Precursors

• DOI: 10.1534/genetics.116.193813
• Publication Date: 2017-03-02T02:10:37Z
• Authors: Kumar Vishal; David S Brooks; Simranjot Bawa; Samantha Gameros; Marta Stetsiv; Erika R Geisbrecht
• Keywords: Armadillo Domain Proteins; Homeodomain Proteins; 0303 health sciences; Gene Expression Regulation, Developmental; Nuclear Proteins; Wnt1 Protein; Muscle Development; Myoblasts; 03 medical and health sciences; Drosophila melanogaster; Gene Knockdown Techniques; Larva; Animals; Drosophila Proteins; Wings, Animal; Cell Proliferation; Signal Transduction; Transcription Factors

Adult muscle precursor (AMP) cells located in the notum of larval wing disc undergo rapid amplification and eventual fusion to generate Drosophila melanogaster indirect flight muscles (IFMs). Here we find that loss Moleskin (Msk) function these disc-associated myoblasts reduces overall AMP pool size, resulting absence IFM formation. This myoblast is due a decrease proliferative capacity independent cell death. In contrast, disruption Msk during pupal proliferation does not alter number, suggesting specifically required for proliferation. It has been previously shown Wingless (Wg) signaling maintains expression Vestigial (Vg) transcription factor proliferating myoblasts. However, other factors influence Wg-mediated are largely unknown. examine interactions between Wg pathway regulation size. We myoblast-specific reduction results Vg complete readout β-catenin/Armadillo (Arm). Moreover, msk RNA interference knockdown abolishes target Ladybird (Lbe) leg Collectively, our provide strong evidence acts through control size formation by regulating Arm stability or nuclear transport.