Response surface optimisation of enzymatically hydrolysed and dilute acid pretreated oil palm trunk bagasse for succinic acid production

• DOI: 10.15376/biores.14.1.1679-1693
• Publication Date: 2022-12-16T15:04:52Z
• Authors: Nurul Adela Bukhari; Jamaliah Md Jahim; Soh Kheang Loh; Nasrin Abu Bakar; Abdullah Amru Ind...
• Keywords: Bagasse; Succinic acid; Central composite design; Enzymatic Hydrolysis; Hemicellulose

The exploitation of agroindustrial lignocellulose, such as oil palm trunk bagasse (OPTB), a raw material in the production succinic acid (SA) may serve an effective strategy to propel bio-based industry. This study aimed optimise recovery fermentable sugar, i.e., glucose, from enzymatic hydrolysis dilute pretreated OPTB (DA-OPTB). pretreatment used this was able remove 59.5% hemicellulose and 13.3% lignin. Response surface methodology (RSM) based on central composite design (CCD) then applied investigate four independent variables – enzyme loading (10 50 U/g), agitation speed (50 250 rpm), reaction time (0 96 h), surfactant concentration (0.025 0.125%, v/v). experimental glucose 21.7 g/L good agreement with RSM-predicted value 20.5 g/L. Among parameters investigated, supplementation during significant influencing recovery, while extent least influential. maximum recovered estimated at 217 g per kg OPTB, 7.3 SA attainable fermented DA-OPTB hydrolysate using Actinobacillus succinogenes 130Z. results demonstrated that can be practically utilised economical high value-added SA.