Disruption of intestinal barrier and dysbiosis of gut microbiota in an experimental rhesus macaque model with 6-year diabetes mellitus

• DOI: 10.1538/expanim.24-0125
• Publication Date: 2025-01-16T22:12:29Z
• Authors: Xiangyu FU; Xiang REN; Maoyuan ZHAO; Lan LI; Yaojia ZHOU; Yanrong LU; Chengshi WANG
• Keywords: Dysbiosis; Rhesus macaque

This study aims to clarify the disruption of gut barrier and dysbiosis microbiota in an experimental macaque model with 6-year diabetes mellitus (DM), provide evidence for application therapeutic strategies targeting human future. A single intravenous injection high-dose streptozotocin was used induce type 1 (T1D) model. Hematoxylin-Eosin (HE) Periodic Acid Schiff (PAS) staining were conducted observe colon morphological changes. The composition detected using 16S rRNA gene sequencing, bioinformatics analysis adopted predict alterations microbial phenotype function. Obvious intestinal inflammation decreased goblet cells observed T1D macaques. sequencing suggested a significantly different β diversity group, where expanded Proteobacteria (dominantly Escherichia-Shigella) Actinomycetota (formerly known as Actinobacteria) replaced dominance Bacillota Firmicutes) Bacteroidota Bacteroidetes), indicating imbalance composition. Archaea identified biomarker between groups. Moreover, reduction beneficial bacteria (Lactobacillaceae) increase pro-inflammatory opportunistic pathogens (Enterobacteriaceae), phenotypes reversed, resulting abnormal up- (e.g., carbohydrate amino acid metabolism) or down-regulation protein digestion absorption) multiple metabolic pathways. There structural disorders macaques, that may be effective treat diseases like DM.