Screening and analysis of metabolic components in rat plasma, urine, and feces after oral administration of Baihe Zhimu Tang by HPLC-ESI-MS methods

• DOI: 10.1556/achrom.23.2011.2.9
• Publication Date: 2011-06-09T01:18:32Z
• Authors: K. M. Qin; Y. Shi; Q. B. Fang; H. Cai; G. M. Yang; B. C. Cai
• Keywords: 0303 health sciences; 03 medical and health sciences; 6. Clean water; 3. Good health

Summary Baihe Zhimu Tang (BZT) is a widely used traditional Chinese medicinal formula for the treatment of various diseases; however, its active components have remained unknown. In this study, high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) methods were developed for the analysis of metabolic components of BZT. The HPLC experiments used a reversed-phase Shim-Pack VP-ODS C18 column with the column temperature at 35°C and a mobile phase system consisting of aqueous formic acid (0.05%, υ/υ) and acetonitrile using a gradient elution at the flow rate of 0.2 mL/min−1. The ESI-MS was operated with a single-quadrupole mass spectrometer in both negative and positive ion modes. After oral administration of extraction of BZT, the rat's plasma, urine, and feces were also analyzed; 37 metabolic components including 23 original components and 14 transformative components of BZT were detected. The analysis of metabolic components in BZT by HPLC-ESI-MS methods could be a us...