Insights into Leptocybe invasa resistance in Eucalyptus: phenotyping, genotyping and in silico approaches.

• DOI: 10.1590/1519-6984.279850
• Publication Date: 2024-01-01
• Authors: Crislaine Costa C...; G.S. Pereira; Juliana Lopes Souza; Valdinete Vieira ...; Sheila Valéria Ál...; José Oliveira Dantas; Genésio Tâmara Ri...; Renata Silva-Mann

The gall wasp, Leptocybe invasa, poses a significant global threat to Eucalyptus cultivation, by causing substantial economic losses. objective of this study was differentiate between resistant and susceptible genotypes morphological characteristics using image analysis based on the damage caused wasp. In addition, consensus sequences derived from transposable elements (TEs) genome spp. Were identified in silico analysis. Furthermore, another discriminate response invasa conducting molecular analyses involving inter simple sequence markers. For analysis, GroundEye ® system used collect images 60 leaves six genotypes, three which were susceptible. obtained GenBank database pairwise alignments with TE conducted BLASTN. Multiple alignment performed Clustal Omega, followed identification conserved regions Jalview. A motif signature generated Weblogo. characterization ISSR markers TEs, samples young total 80 seedlings, 50 classified as 30 L. invasa. It possible distinguish wasp enabled genome, associated proteins involved secondary metabolite production, e.g., terpenes, play role discrimination capacity TEs primers demonstrated bands that could be identify genotypes. However, increasing number required both cases is suggested.