Medication-related osteonecrosis of the jaw. Introduction of a new modified experimental model

• DOI: 10.1590/s0102-865020160050000003
• Publication Date: 2016-06-04T16:20:55Z
• Authors: Cláudia Curra; Camila Lopes Cardoso; Osny Ferreira Júnior; Marcos Martins Curi; Mariza Akemi Mats...; Bruno Cavalini Ca...; Pâmela Letícia do...; Joel Ferreira San...
• Keywords: Male; RD1-811; Alveolar Bone Loss; Osteolysis; Zoledronic Acid; 630; 03 medical and health sciences; 0302 clinical medicine; Animals; Rats, Wistar; Osteitis; Diphosphonates; Bisphosphonate-associated osteonecrosis of the jaw; Imidazoles; Rats.; X-Ray Microtomography; Maxillary Diseases; Rats; 3. Good health; Incisor; Models, Animal; Tooth Extraction; Surgery; Administration, Intravenous; Bisphosphonate-Associated Osteonecrosis of the Jaw

To evaluate a modified experimental model for medication-related osteonecrosis of the jaw (MRONJ) through upper right central incisor extraction followed by intravenous bisphosphonate administration.Forty five rats underwent tooth were divided in 2 groups: Group I - group, 30 received an administration protocol zoledronic acid 35μg/kg into tail vein every two weeks, totalizing four administrations, during eight weeks administration, previously extraction, and II control 15 didn't any medication before extraction. The groups subdivided postoperative periods: 14/28/42 days. Clinical analysis microtomography performed to verify presence osteonecrosis. In addition, descritive histological hematoxylin-eosin stained sections was or necrotic foci.Twelve (40%) rats, from showed clinical signs MRONJ (p=0.005), however, all samples imaginologic findings like osteolysis loss integrity cellular walls (p≤0.001). Microscopic evaluation revealed areas with microbial colonies inflammatory infiltrate animals presented chronology normal wound healing.The after maxillary rats. This new may be considered option study MRONJ.