Inhibitory Activity of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) on Transformed Cells by Human Papillomavirus

• DOI: 10.1615/intjmedmushr.v16.i2.80
• Publication Date: 2014-05-05T23:31:06Z
• Authors: Eva Hernandez-Mar...; Alfredo Lagunas-M...; Victor H. Bermude...; Ana I. Burgete-Ga...; Ismael Leon-Rivera; Elizur Montiel-Arcos; Enrique Garcia-Villa; Patricio Gariglio; Vicente Madrid-Ma...; Raul N. Ondarza-V...
• Keywords: HeLa; MTT assay; Viability assay; Trypan blue

In this study, we investigated the effects of aqueous extracts Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum, obtained from three localities (China; and Morelos Michoacan, Mexico) on cervical cells transformed by human papillomavirus (HeLa SiHa) C-33A cancer cells. The were plated in DMEM medium supplemented, incubated presence different concentrations G. lucidum for 24 h. Cell proliferation was determined MTT colorimetric assay viability trypan blue assay. Inhibitory dose (IC50) culture cell lines mentioned above. apoptosis process confirmed nuclear DNA fragmentation cycle flow cytometry. results showed that produced inhibition VPH cells; they also induced arrest HeLa, SiHa, Therefore, it found locations inhibitory effect may have a potential therapeutic use prevention treatment disease.