Role of CD34 Antigen in Myeloid Differentiation of Human Hematopoietic Progenitor Cells

• DOI: 10.1634/stemcells.2007-0597
• Publication Date: 2008-01-12T01:15:13Z
• Authors: Simona Salati; Roberta Zini; Elisa Bianchi; Anna Testa; Fulvio Mavilio; Rossella Manfredini; Sergio Ferrari
• Keywords: 0303 health sciences; Gene Expression Profiling; Antigens, CD34; Cell Differentiation; Hematopoietic Stem Cells; Clone Cells; 03 medical and health sciences; Humans; Gene Silencing; Cells, Cultured; Myeloid Progenitor Cells; CD34+ cells; CD34 antigen; myeloid differentiation; erithropoiesis; megacaryocytopoiesis; Oligonucleotide Array Sequence Analysis

Abstract CD34 is a transmembrane protein that strongly expressed on hematopoietic stem/progenitor cells (HSCs); despite its importance as marker of HSCs, function still poorly understood, although role in cell adhesion has been demonstrated. To characterize the antigen human we examined, by both inhibition and overexpression, regulation HSC lineage differentiation. Our results demonstrate silencing enhances granulocyte megakaryocyte differentiation reduces erythroid maturation. In agreement with these results, gene expression profile reveals upregulation genes involved downregulation genes. Consistently, retroviral-mediated overexpression leads to remarkable increase progenitors dramatic decrease progenitors, evaluated clonogenic assay. Together, data indicate molecule promotes CD34+ toward lineage, which achieved, at least part, expense lineages. Disclosure potential conflicts interest found end this article.