Characterization of Nephropathogenic Infectious Bronchitis Virus DMV/1639/11 Recovered from Delmarva Broiler Chickens in 2011

• DOI: 10.1637/10313-080212-reg.1
• Publication Date: 2013-04-01T22:44:34Z
• Authors: Jack Gelb; Brian S. Ladman; Conrad R. Pope; J. Miguel Ruano; Erin M. Brannick; Daniel A. Bautista; Colleen M. Coughlin; Lauren A. Preskenis
• Keywords: Flock

A limited outbreak of nephropathogenic infectious bronchitis (NIB) occurred in three Delmarva (DMV) commercial broiler chicken flocks 2011. Isolates NIB virus (NIBV)—DMV/1639/11, DMV/3432/11, and DMV/3902/11—were characterized by sequence analysis the N-terminal subunit (S1) spike (S) gene. Findings indicated that isolates were identical to each other PA/9579A/10, a 2010 isolate from poultry Pennsylvania. The 2011 appear have originated 1997–2000 DMV/1639/11 PA/9579A/10 determined be susceptible chickens, yielding reisolations kidney inducing characteristic interstitial nephritis microscopic lesions. In controlled laboratory study, 40% chickens vaccinated with combination live vaccine containing (IBV) strains Massachusetts (Mass) Connecticut (Conn) positive on isolation attempts after challenge DMV/1639/11, compared only 13% Mass Arkansas (Ark) vaccinates. Both vaccines gave partial protection against development DMV/1639/11-induced renal Although numerically fewer Conn had those Ark, neither offered greater (P < 0.05) than observed unvaccinated challenged DMV/1639/11. Ark vaccinations, applied under conditions hatchery (spray) on-farm (spray), did not protect trachea or challenge. Serologic testing found <3% (2 69) tested possess specific antibodies indicating become established region.Caracterización del nefropatogénico de la bronquitis infecciosa aislado pollos engorde en el área año 2011.Se presentó un brote limitado nefropatogénica tres parvadas comerciales Delmarva. Se caracterizaron los aislamientos nefropatogénicos DMV/3432/11 y DMV/3902/11 se mediante análisis secuencia gene subunidad S1 espícula (S). Los resultados indicaron que eran idénticos entre sí con aislamiento es aves Pennsylvania 2010. Las cepas años parecen haberse originado partir 1997 al 2000 determinó susceptibles, reaislados riñón e inducían las lesiones nefritis intersticial microscópica característica. En estudio controlado laboratorio, vacunados una vacuna viva contenía combinación Massachussets fueron positivos intentos después desafío comparación sólo (Ark). Ambas combinaciones vacunas confirieron protección parcial contra desarrollo renales inducidas por Aunque número menor mostraron Arkansas, ninguna ofreció mayor observada no desafiados vacunaciones aplicadas condiciones planta incubación (aerosol) explotaciones agrícolas (aerosol), protegieron tráquea o lo