GENETIC RELATIONSHIPS AMONG ANISAKIS SPECIES (NEMATODA: ANISAKIDAE) INFERRED FROM MITOCHONDRIALCOX2SEQUENCES, AND COMPARISON WITH ALLOZYME DATA

• DOI: 10.1645/ge-3504.1
• Publication Date: 2006-07-16T20:23:43Z
• Authors: Alice Valentini; Simonetta Mattiucci; Paola Bondanelli; Stephen C. Webb; Antonio A. Mignuc...; Marlene M. Colom-...; Giuseppe Nascetti
• Keywords: Anisakidae; Anisakis; Anisakis simplex; Maximum parsimony

The genetic relationships among 9 taxa of Anisakis Dujardin, 1845 (A. simplex (sensu stricto), A. pegreffii, C., typica, ziphidarum, physeteris, brevispiculata, paggiae, and sp.) were inferred from sequence analysis (629 bp) the mitochondrial cox2 gene. Genetic divergence considered taxa, estimated by p-distance, ranged p = 0.055, between sibling species complex, to 0.12, morphologically differentiated species, i.e., ziphidarum typica. highest level was detected when comparing paggiae versus complex (on average 0.13) or typica 0.14). Sequence data newly identified sp. poorly aligned with other but most similar (p 0.08). Phylogenetic analyses based upon Parsimony Bayesian Inference, as well phenetic Neighbor-Joining p-distance values, generated tree topologies, each supported at major nodes. All delineated two main claides, first encompassing a sister group all remaining second comprising (s.s.), pegreffii C), In general, mtDNA-based topologies showed high congruence those nuclear sets (19 enzyme-loci) morphological delineating adult larval stages spp.; however, precise positioning remain resolved, though they consistently clustered in same clade complex. Comparison anisakid currently available for their cetacean-definitive hosts suggests parallelism host parasite phylogenetic topologies.