Effects of glucose and insulin on glucokinase activity in rat hypothalamus

• DOI: 10.1677/joe-06-0146
• Publication Date: 2007-04-30T14:29:53Z
• Authors: Carmen Sanz; Isabel Roncero; Patricia Vázquez; M Angeles Navas; Enrique Blázquez
• Keywords: Glucokinase

In an attempt to study the role of glucokinase (GK) and effects glucose peptides on GK gene expression activity this enzyme in hypothalamus, we used two kinds biological models: hypothalamic GT1-7 cells rat slices. The was reduced by insulin (INS) not modified different concentrations, while activities were significantly peptides. Interestingly, a distinctive pattern between ventromedial hypothalamus (VMH) lateral (LH) found, with higher VMH as concentrations rose, LH decreased at 2.8 20 mM glucose, latter effect being prevented incubation INS. These produced only d-glucose modifications found due GK, but other hexokinases. addition, glucagon-like peptide 1, leptin, orexin B, INS, neuropeptide Y (NPY), statistically significant for NPY LH. Our results indicate that both occur rather than transcription. Moreover, INS suggest brain behaves manner opposite liver, which might facilitate its sensing. Finally, slices seem offer good physiological model discriminate areas.