Expression of prolactin mRNA and of prolactin-like proteins in endothelial cells: evidence for autocrine effects

• DOI: 10.1677/joe.0.1580137
• Publication Date: 2004-12-02T23:42:13Z
• Authors: C Clapp; FJ Lopez-Gomez; G Nava; A Corbacho; L Torner; Y Macotela; Z Duenas; A Ochoa; G Noris; E Acosta; E Garay; G Martinez de la ...
• Keywords: Prolactin cell

Formation of new capillary blood vessels, termed angiogenesis, is essential for the growth and development tissues underlies a variety diseases including tumor growth. Members prolactin hormonal family bind to endothelial cell receptors have direct effects on proliferation, migration tube formation. Because many angiogenic antiangiogenic factors are produced by cells, we investigated whether cells expressed gene. Here show that bovine brain (BBCEC) in culture express full-length messenger RNA, addition novel transcript, lacking third exon In cultures BBCEC synthesize secrete prolactin-like immunoreactive proteins with apparent molecular masses 23, 21 14 kDa. The nature these supported observation Nb2-cells, prolactin-responsive line, were stimulated proliferate when co-cultured this stimulation was neutralized prolactin-directed antibodies. Finally, consistent possible autocrine effect endothelial-derived prolactins, polyclonal monoclonal antibodies specifically inhibited basal basis fibroblast growth-factor-stimulated cells. Taken together, present findings support hypothesis gene being as could act an fashion regulate proliferation.