Inflammation and nitric oxide production in skeletal muscle of type 2 diabetic patients

• DOI: 10.1677/joe.0.1810419
• Publication Date: 2004-12-02T23:42:13Z
• Authors: SH Torres; JB De Sanctis; L M de Briceno; N Hernandez; HJ Finol
• Keywords: Nitrotyrosine; CD163

An inflammatory process may be involved in nitric oxide production skeletal muscle of type 2 diabetic patients. Nitric generation was assessed 14 non-complicated patients and 12 healthy subjects. In samples quadriceps femoris muscle, endothelial synthase (eNOS), inducible (iNOS), nitrite, nitrate nitrotyrosine were determined. The macrophage-specific antigen CD163, the T-cell membrane factor CD154 tumour necrosis factor-alpha (TNF-alpha) also assayed. six patients, ultrastructural analysis performed. Nitrites nitrates increased as compared to controls (22.7+/-4.5 32.7+/-7.0 vs 16.0+/-2.9 22.8+/-4.0 micromol/mg protein; P<0.001, Mann-Whitney U test). Endothelial NOS similar control subjects (36.4+/-13.8 36.3+/-6.8 ng/mg protein), contrasting with significant increase iNOS recorded (34.3+/-13.0 8.5+/-2.8 protein, P<0.00002). Nitrotyrosine levels higher patient than group (42.1+/-24.4 10.3+/-2.5 P<0.00002), CD163 (10-fold) TNF-alpha (fourfold) levels. Furthermore, detectable only (10.2+/-5.3 protein). By multiple-regression analysis, changes glycated haemoglobin values could predict 96% variation nitrotyrosine. Macrophages present all analysed by electromicroscopy. indicate that an occurs This contribute induction, damage insulin resistance.