Enzymatic Conversion of Sugar Beet Pulp: A Comparison of Simultaneous Saccharification and Fermentation and Separate Hydrolysis and Fermentation for Lactic Acid Production

• DOI: 10.17113/ftb.56.02.18.5390
• Publication Date: 2018-02-22T10:41:46Z
• Authors: Joanna Berlowska; Weronika Cieciura...; ; Halina Kalinowska; ; Dorota Kregiel; ; Sebastian Borowski; ; Ewelina Pawlikowska; ; Michal Binczarski; ; Izabela Witonska
• Keywords: Beet pulp; Enzymatic Hydrolysis

This study compares the efficiency of lactic acid production by separate hydrolysis and fermentation (SHF) or simultaneous saccharification (SSF) sugar beet pulp, a byproduct industrial production. In experiments, pulp was hydrolyzed using five commercial enzymes. A series shake flask fermentations were conducted selected strains bacteria (LAB). The differences in activities enzymes for degrading principal components reflected different yields total reducing sugars. highest after lowest quantities insoluble residues obtained mixture (1:1) Viscozyme® Ultraflo® Max. SHF process, only portion soluble sugars released from assimilated LAB strains. SSF, low enzyme loads led to reduction accumulation. risk carbon catabolic repression reduced. Our results suggest that SSF has advantages over SHF, including lower processing costs higher productivity. Lactic yield mode (approx. 30 g/L) 80-90% than SHF.