Protocol for DNA extraction and shotgun metagenomic sequencing for the study of the mice digestive microbiota v1

• DOI: 10.17504/protocols.io.bp2l6x5wklqe/v1
• Publication Date: 2025-02-12T21:59:46Z
• Authors: Victoria Meslier; Mallia Geiger; Yani Ren; Alexandre Famechon; Marine Gilles; Aymeric David; Christian MORABITO; Benoit Quinquis; Mathieu Almeida
• Keywords: Shotgun; Pyrosequencing

This protocol describes the procedures for whole DNA extraction, high throughput sequencing, metagenomic read mapping and bioinformatical pre-processing to determine microbial composition functional potential of mice digestive microbiota.