Neutrophils as a source of factors that increase the length of the inflammatory phase of wound healing in patients with type 2 diabetes mellitus

• DOI: 10.18097/pbmc20186405433
• Publication Date: 2018-10-31T07:00:08Z
• Authors: E.V. Mikhalchik; D.I. Maximov; E.M. Ostrovsky; A.V. Yaskevich; I.I. Vlasova; T.V. Vakhrusheva; L.Yu. Basyreva; A.A. Gusev; V.A. Kostevich; N.P. Gorbunov; A.V. Sokolov; O.M. Panasenko; S.A. Gusev
• Keywords: 0301 basic medicine; Wound Healing; 03 medical and health sciences; Diabetes Mellitus, Type 2; Neutrophils; Humans; Extracellular Traps; Peroxidase; Retrospective Studies; 3. Good health

Oxidative stress and neutrophil activation leading to an increase in myeloperoxidase (MPO), elastase extracellular trap (NET) levels blood are considered as pathogenic mechanisms responsible for the development of extremity damage people with type 2 diabetes mellitus (T2DM). The aim this study was analyze relationship between factors, associated activation, length initial phase wound healing (the inflammatory phase) T2DM patients. Patients were divided retrospectively into three groups depending on extent: group 1 (wound toe) < foot) 3 lower leg). Compared control (healthy volunteers), patients at admission hospital had significantly (p<0.05) increased glucose glycated hemoglobin (groups 1-3), ESR 3), count plasma MPO concentration 1-3) NET (group 3) decreased thiols erythrocyte glutathione peroxidase activity 3). stay after surgical procedures corresponded process correlated number neutrophils before surgery (r=0.72, p<0.05). Leukocytic intoxication index depended area (r=0.59, p<0.05), it higher compared 1. extremities healing. correlation found can be attributed NETs, which, its turn, results from degranulation netosis. Thus, duration depends specific aspects systemic inflammation increasing oxidative/halogenative intoxication.Okislitel'nyĭ i aktivatsiiu neĭtrofilov, vedushchuiu k povysheniiu soderzhaniia v krovi mieloperoksidazy élastazy neĭtrofil'nykh vnekletochnykh lovushek (NVL), otnosiat patogeneticheskim mekhanizmam povrezhdeniia konechnosteĭ pri sakharnom diabete 2-go tipa (SD2). Tsel'iu issledovaniia byl analiz sviazi mezhdu faktorami, kharakterizuiushchimi dlitel'nost'iu nachal'noĭ fazy zazhivleniia rany (fazy vospaleniia) u bol'nykh SD2. Retrospektivno patsienty byli razdeleny na tri gruppy zavisimosti ot tiazhesti porazheniia nizhnikh konechnosteĭ: gruppa (rana pal'tse) stope) goleni). Po sravneniiu s kontrol'noĭ gruppoĭ (zdorovye dobrovol'tsy) patsientov postuplenii lechenie dostoverno (r<0,05) povysheny uroven' gliukozy glikirovannogo gemoglobina (gruppy SOÉ soderzhanie neĭtrofilov kontsentratsiia plazme NVL (gruppa a takzhe snizheno tiolov plazmy aktivnost' glutationperoksidazy éritrotsitov Prodolzhitel'nost' prebyvaniia statsionare posle operatsii sootvetstvovala dlitel'nosti vospaleniia ranevogo protsessa korrelirovala soderzhaniem do nachala lecheniia (r=0,72, r<0,05). Leĭkotsitarnyĭ indeks intoksikatsii zavisel ploshchadi (r=0,59, r<0,05) gruppakh vyshe, chem kontrole gruppe Vyiavlennaia korreliatsiia obuslovlena rostom vnekletochnoĭ rezul'tate aktivatsii degranuliatsii netoza. Takim obrazom, dlitel'nost' zazhivlenii sviazana osobennostiami sistemnogo vospaleniia, vedushchego usileniiu okislitel'nogo/galogeniruiushchego stressa éndogennoĭ intoksikatsii.