cAMP сoncentrations in cardiac mitochondria and serum in the С57ВL/6 mice under independent melanoma В16/F10 growth versus melanoma В16/F10 growth linked to chronic neurogenic pain

• DOI: 10.18137/cardiometry.2022.22.6268
• Publication Date: 2022-06-17T14:04:20Z
• Authors: O.I. Kit; E.M. Frantsiyants; V.A. Bandovkina; I.V. Neskubina; A.I. Shikhlyarova; I.V. Kaplieva; E.I. Surikova; Y.A. Pogorelova; N.D. Cheryarina; L.K. Trepitaki; I.A. Goroshinskaya; L.N. Vaschenko; Y.S. Shatova; L.P. Kuchkina; V.A. Kovalenko; L.A. Nemashkalova
• Keywords: Strain (injury)

The aim of this research work is to study the cAMP level in cardiac mitochondria and serum С57ВL/6 strain mice both genders under independent melanoma В16/F10 growth versus linked chronic neurogenic pain (CNP). Materials methods. Mice (n=336) have been grouped as follows: intact group (♂n=21; ♀n=21), reference ♀n=21) with reproduced CNP model, comparison (♂n=63; ♀n=63) include В16/F10, main test cover against background. Upon expiration 1 week, 2 3 weeks growth, animals above experimental groups isolated centrifugation using high-performance refrigerated centrifuge Avanti J-E, BECMAN COULTER, USA. With ELISA Kit (RayBio USA) we determined concentrations mitochondria. Results. has induced a decrease by factor 3,6 female only. In heart increasing beginning week tumor on average 4, while starting from it recorded 2-4 times higher was depleted end experiment. As concentration serum, dynamics thereof not found be correlation mitochondrial data, its females males. Conclusion. So, changes demonstrate their gender-specific feature; males responded an impact produced CNP. group, stimulated increase earlier than case resulted full depletion 3rd