Limited Long-Term Impact of Insect Venom Immunotherapy on the Micro-RNA Landscape in Whole Blood

• DOI: 10.18176/jiaci.0303
• Publication Date: 2018-09-05T15:33:26Z
• Authors: P Karpinski; M Kahraman; N Ludwig; P Skiba; M Kosinska; M Rosiek-Biegus; E Królewicz; B Panaszek; M Nittner-Marszalska; N Blin; A Keller; E Meese; MM Sasiadek
• Keywords: Allergic reaction

To perform a genome-wide characterization of changes in microRNA (miRNA) expression during the course venom immunotherapy (VIT).miRNA was isolated from whole-blood 13 allergic patients and 14 controls, who experienced no reaction upon stings by honeybees wasps. We analyzed 2549 miRNAs whole blood these prior to VIT 12 months after start VIT. The results for differential obtained on microarray platform were confirmed quantitative real-time PCR. Out patients, 8 had negative with VIT, thus indicating that this approach successful.By comparing time points before ultrarush correlation analysis principal component both indicated limited effect overall miRNA pattern. Volcano plot based raw P values revealed few deregulated miRNAs, most which increasingly expressed as compared Based 50 altered clear clustering observed or VIT.Our indicate an reduced pattern blood.