Targeting long non-coding RNA MALAT1 alleviates retinal neurodegeneration in diabetic mice

• DOI: 10.18240/ijo.2020.02.03
• Publication Date: 2020-02-06T09:55:46Z
• Authors: Yu-Lan Zhang
• Keywords: MALAT1; Outer nuclear layer

To observe the effect of inhibiting long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on diabetic neurodegeneration.Thirty-six 8-week-old C57BL/6 mice were randomly divided into normal control, scrambled small interfering RNAs (siRNAs) and MALAT1-siRNA groups. After induction with streptozocin intraperitoneally-injection, group was intravitreally injected µL 20 µmol/L MALAT1 siRNA, siRNA same amount siRNA. Electroretinography performed to examine photoreceptor functions 16wk after diabetes induction. expression detected via real time polymerase chain reaction. Cone morphological changes examined using immunofluorescence. Rod by determining outer nuclear layer (ONL) thickness.The upregulation retinal in control mice, while decreased 91.48% compared mice. The showed lower a-wave b-wave amplitudes than did scotopic photopic electroretinogram, higher Morphological examination revealed that ONL thickness However, greater Moreover, a sparser cone cell arrangement shorter segment morphology mice.Inhibiting results mitigative effects photoreceptors, thus alleviating neurodegeneration.