Small extracellular vesicles and their miRNA cargo are anti-apoptotic members of the senescence-associated secretory phenotype

• DOI: 10.18632/aging.101452
• Publication Date: 2018-05-19T17:58:46Z
• Authors: Lucia Terlecki-Za...; Ingo Lämmermann; Julie Latreille; Madhusudhan Reddy...; Vera Pils; Markus Schosserer; Regina Weinmüllner; Hanna Dellago; Susanna Skalicky; Dietmar Pum; Juan Carlos Higar...; Marcel Scheideler; Frédérique Morizot; Matthias Hackl; Florian Gruber; Johannes Grillari
• Keywords: Senescence

Loss of functionality during aging cells and organisms is caused accompanied by altered cell-to-cell communication signalling. One factor thereby the chronic accumulation senescent concomitant senescence-associated secretory phenotype (SASP) that contributes to microenvironment remodelling a pro-inflammatory status. While protein based SASP factors have been well characterized, little known about small extracellular vesicles (sEVs) their miRNA cargo. Therefore, we analysed secretion sEVs from human dermal fibroblasts catalogued therein contained miRNAs. We observed four-fold increase sEVs, with >80% all cargo The most abundantly secreted miRNAs were predicted collectively target mRNAs pro-apoptotic proteins, indeed, cell derived exerted anti-apoptotic activity. In addition, identified senescence-specific differences in composition an miR-23a-5p miR-137 decrease miR-625-3p, miR-766-3p, miR-199b-5p, miR-381-3p, miR-17-3p. By correlating intracellular sEV-miRNAs, selectively retained (miR-21-3p miR-17-3p) or packaged specifically into (miR-15b-5p miR-30a-3p). suggest be novel, members are cellular senescence.