N-acetyl-L-cysteine (NAC) delays post-ovulatory oocyte aging in mouse

• DOI: 10.18632/aging.101898
• Publication Date: 2019-04-12T16:07:59Z
• Authors: Yue Wang; Li Li; Li-Hua Fan; Ying Jing; Jian Li; Ying-Chun Ouyang; Zhen-Bo Wang; Yi Hou; Qing-Yuan Sun
• Keywords: Ovulation; Oocyte; Aging; Mice, Inbred ICR; 0303 health sciences; Post-ovulatory aging; ROS; Spindle Apparatus; Cytoplasmic Granules; Time-Lapse Imaging; Antioxidants; Acetylcysteine; Mitochondria; Mice; 03 medical and health sciences; Oocytes; Animals; Female; Reactive Oxygen Species; Cellular Senescence; Research Paper

The quality of post-ovulatory oocytes decreases with aging. In this study, we aimed to investigate the effects N-acetyl-L-cysteine (NAC), a broadly used antioxidant, on oocyte in mouse aging vitro. NAC at 0.6mM concentration was added culture medium (M2), and analyzed 6h, 12h, 18h 24h culture. We found that frequency spindle defects decreased NAC-treated compared those without treatment. treatment significantly abnormal distribution cortical granules (CGs) during for 24h. Decreased intracellular reactive oxygen species (ROS) also observed. Increased ATP levels mitochondria could be observed supplementation These results indicate will maintain oocytes, delay as studied mouse.