The acute transcriptional response to resistance exercise: impact of age and contraction mode

• DOI: 10.18632/aging.101904
• Publication Date: 2019-04-15T19:39:09Z
• Authors: Colleen S. Deane; Ryan M. Ames; Bethan E. Phillips; Michael N. Weedon; Craig R.G. Willis; Catherine Boereboom; Haitham Abdulla; Syed S.I. Bukhari; Jonathan N. Lund; John P. Williams; Daniel J. Wilkinson; Kenneth Smith; Iain J. Gallagher; Fawzi Kadi; Nathaniel J. Szew...; Philip J. Atherton; Timothy Etheridge
• Keywords: Vastus lateralis muscle

Optimization of resistance exercise (RE) remains a hotbed research for muscle building and maintenance.However, the interactions between contractile components RE (i.e.concentric (CON) eccentric (ECC)) age, are poorly defined.We used transcriptomics to compare age-related molecular responses acute CON ECC exercise.Eight young (21±1 y) eight older (70±1 exercise-naïve male volunteers had vastus lateralis biopsies collected at baseline 5 h post unilateral contralateral exercise.RNA was subjected next-generation sequencing differentially expressed (DE) genes tested pathway enrichment using Gene Ontology (GO).The transcriptional response highly similar adults displayed moderate contraction-specific profiles, with no GO enrichment.Age-specific revealed 104 DE unique young, 170 in muscle, enrichment.Following CON, 15 were muscle-specific, whereas uniquely 147 up-regulated enriched cell adhesion blood vessel development, 28 down-regulated involved mitochondrial respiration, amino acid lipid metabolism.Thus, age is associated regulation often without clear functional relevance, perhaps reflecting degree stochastic dysregulation.