Electroacupuncture ameliorates cerebral ischemia/reperfusion injury by suppressing autophagy via the SIRT1-FOXO1 signaling pathway

• DOI: 10.18632/aging.103420
• Publication Date: 2020-07-04T03:36:35Z
• Authors: Zhi-Gang Mei; Ya-Guang Huang; Zhi-Tao Feng; Ya-Nan Luo; Song-Bai Yang; Li-Peng Du; Kang Jiang; Xiao-Lu Liu; Xian-Yun Fu; Yi-Hui Deng; Hua-Jun Zhou
• Keywords: FOXO1; Zusanli

Cerebral ischemia/reperfusion (CIR) injury occurs when blood flow is restored in the brain, causing secondary damage to ischemic tissues. Previous studies have shown that electroacupuncture (EA) treatment contributes brain protection against CIR through modulating autophagy. Studies indicated SIRT1-FOXO1 plays a crucial role regulating Here we investigated mechanisms underlying neuroprotective effect of EA and its autophagy via signaling pathway rats with injury. pretreatment at "Baihui", "Quchi" "Zusanli" acupoints (2/15Hz, 1mA, 30 min/day) was performed for 5 days before were subjected middle cerebral artery occlusion, results substantially reduced Longa score infarct volume, increased dendritic spine density lessened autophagosomes peri-ischemic cortex rats. Additionally, also ratio LC3-II/LC3-I, levels Ac-FOXO1 Atg7, interaction but p62, SIRT1, FOXO1. The above effects abrogated by SIRT1 inhibitor EX527. Thus, presume elicits injury, potentially suppressing activating pathway.