Hair follicle mesenchymal stem cell exosomal lncRNA H19 inhibited NLRP3 pyroptosis to promote diabetic mouse skin wound healing

• DOI: 10.18632/aging.204513
• Publication Date: 2023-02-15T16:32:33Z
• Authors: Hongliang Yang; Yan Zhang; Zhenwu Du; Tengfei Wu; Chun Yang
• Keywords: HaCaT; Pyroptosis; Skin repair; Exosome

Skin wounds caused by diabetes are a major medical problem. Mesenchymal stem cell-derived exosomes hold promise to quicken wound healing due their ability transfer certain molecules target cells, including mRNAs, microRNAs, lncRNAs, and proteins. Nonetheless, the specific mechanisms underlying this impact not elucidated. Therefore, research aimed investigate effect of MSC-derived comprising long non-coding RNA (lncRNA) H19 on diabetic skin healing. Hair follicle mesenchymal cells (HF-MSCs) were effectively isolated detected, (Exo) also smoothly. Pretreatment with 30 mM glucose for 24 h (HG) could efficiently induce pyroptosis in HaCaT cells. Exosomal enhanced proliferation migration inhibited reversing stimulation NLRP3 inflammasome. Injection overexpressing lncRNA promoted sustained healing, whereas sh-H19 did have effect. In conclusion, Exosomes proliferation, suppressed both vitro vivo. HFMSC-derived that overexpress may be included strategies wounds.